Fig. 2. Effect of ChR on EMT and CSLC features in HeLa cells induced by TNF-α combined with TGF-β. HeLa cells were co-treated with TNF-α and TGF-β with or without different concentrations (5.0, 10.0 and 20.0μM) of ChR. ChR reversed mesenchymal morphology (A, scale bar, 50 μm), regulated E-cadherin and N-cadherin (B), reduced nuclear translocation of NF-κBp65 and Twist1(C), inhibited cell migration (D) and self-renewal (E, scale bar, 200 μm) abilities, and downregulated Bmi1, Sox2 and Oct4 (F) as well as CD133, CD44 and ALDH1 (G) at the protein level.^*p<0.05, vs 0.1%DMSO treatment; ^#p<0.05, vs ChR (10μM) treatment. (C) HeLa cells co-treated with TNF-α and TGF-β were treated with ChR (10.0 μM) for the indicated times. Cytosolic and nuclear NF-κBp65 and Twist1 protein were separated using hypotonic buffer. β-actin and lamin B indicate cytosolic and nuclear fraction, respectively.